Chunk #27 — Results — The effects of age, gender, cause of death, region, PMI, brain pH and RIN-based RNA quality on array performance (%P, cDNA and cRNA profile)
Outside of pH and PMI, which for reasons described previously were analysed separately, the most important covariates were brain region (explained 12.4% of the variation in %P, p = 3.6 × 10−53 ), followed by brain bank (explained an additional 4.7%, p = 9.6 × 10−6 ), and then RIN (explained an additional 2.7%, p = 9.1 × 10−05 ). Age, gender, and cause of death together explained an additional 2.2% of the variation. The effect of brain region on %P was most obvious when comparing CRBL and WHMT. CRBL showed highest %P (mean = 68%) whereas WHMT showed the lowest %P (mean = 57.5%) (Fig. 3).
