Chunk #26 — Results — The effects of age, gender, cause of death, region, PMI, brain pH and RIN-based RNA quality on array performance (%P, cDNA and cRNA profile)
We assessed the dependence of RNA array performance quality on RIN-based RNA quality and the other covariates available to us. A systematic quality control check of the arrays was performed using Expression Console™ software. This software produces a number of array quality measures. The most reliable and widely used parameter is the present call (%P) (Tomita et al. 2004; Weis et al. 2007). The percent present call is the percentage of probe sets with signal detection above background probe level p -value of ≤ 0.01. The range of %P in this study was 1.7–77.3% (mean of 61.4%). Thrity-six per cent of the variation in %P was explained by individual-level differences (adjusted R2 measure).
