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Chunk #12 — Methods

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The relationship of 5HTT (SLC6A4) methylation and genotype on mRNA expression and liability to major depression and alcohol dependence in subjects from the Iowa Adoption Studies.
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All data were analyzed using the JMP analytic suite, Version 5.1 (SAS Institute, Cary, SC) using Pearson’s correlation co-efficients or two tailed, regression (analysis of variance, ANOVA, ordinal logistic regression, OLR), Chi-square or t-tests tests as described in the text [Fleiss, 1981]. Symptom counts and 5HTTLPR genotype were treated as ordinal (s,s < s,l < l,l) or nominal variables when indicated. Sex and categorical diagnosis was treated as a nominal variable. Methylation ratios and normalized 5HTT mRNA expression were treated as continuous variables.