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Chunk #10 — Materials and Methods — Coexpression Network Construction

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Regional Differences and Similarities in the Brain Transcriptome for Mice Selected for Ethanol Preference From HS-CC Founders.
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The coexpression network was constructed by means of the WGCNA (Langfelder and Horvath, 2008; Iancu et al., 2012). We started by constructing adjacency network matrices independently for each region by computing the Pearson correlation between all gene pairs. These values were raised to a power β = 6 for all regions, which was chosen such that the network approaches a scale-free structure (exponential distribution of node connectivity).