A number of Sequenom-validated genes were functionally closely related to neural development and neurulation. Altered methylation of key genes relevant to neurons (Elavl2, a Hu antigen expressed in immature neurons62) and glia development (Neuroligin 3, Nlgn3; gain-of-function of Nlgn3 is regarded as a new model for autism spectrum disorders63), neural patterning (Sox21), and neuroendocrine function (Sim1) were observed. Gene expression data was available for Elavl2 and Sox21, and decreased methylation corresponded with increased expression. Furthermore, we validated that Hist1h3d, which encodes a member of the Histone H3 gene family responsible for nucleosome structure, displayed decreased methylation.
