To further probe the altered energy homeostasis that is demonstrated in the Cyp1b1-ko mice, we compared liver gene expression profiles from mice with extensive adipose suppression on HFD to WT and LFD counterparts. Agilent microarray profiles have been analyzed for three individual mice in each of the four treatment groups. Each gene is represented as one of three expression ratios: the mean for WT mice on the HFD is compared to the WT mean on the LFD (designated WT/HFD); the mean for Cyp1b1-ko mice is compared to the mean for WT mice on the same diet (designated KO/LFD and KO/HFD, respectively). The Cy5 binding provides a measure of relative basal gene expression in the WT mice. The LFD/HFD diet change and Cyp1b1 deletion mediated 681 gene responses that differed between the treatment groups by 2-fold or greater, with p<0.05 (Table S2). Remarkably, there was extensive overlap between their effects, with three times more genes responding to Cyp1b1 deletion (KO/LFD or KO/HFD) (560) than to this major diet change in WT mice (WT/HFD) (176). Accordingly, Cyp1b1 expression in the WT mice was not affected by the diet change (WT HFD/LFD=1.22, p=0.625).
