In female mice, the amount of ethanol consumed was significantly reduced in all six null strains. Ctss, Il1rn and CD14 null strains showed reduced preference for ethanol in the 2B-DID test [main effect of genotype: F(1,162) = 5.7, p<0.05 for Ctss mice; F(1,126) = 490, p<0.001 for Il1rn mice; F(1,162) = 9.1, p<0.01 for Cd14 mice] (Fig. 3 d,e,f). Total fluid intake was significantly reduced in B2m, Ctss and Il6 null female mice and increased in Il1rn and Cd14 knockout female mice (for complete results see Supplemental Fig. 6; for detailed statistics see Supplemental Table 8). Some strains appeared to increase their alcohol consumption over days, but this was not a consistent finding for either the wild-type or mutant mice (statistics in Supplemental Table 9). Because Ctsf null mice of both sexes showed very low preference for ethanol in the 24 hr two-bottle choice test, these mice were not tested in either 2B-DID or 1B-DID.
