The aim, with continued curation, is to add sequences from newly described orders and to extend representation to include most of the accepted families with a focus on medically important Fungi. The process also includes making corrections, replacing sequence from verified material with sequence from type material as it becomes available and editing definition lines or removing RefSeq records as taxonomic classifications changes. This ensures that BLAST search results correctly display the current name. The RefSeq ITS records have been extended to represent 3,060 sequences representing 270 families from 39 classes. During the initial collaborative ITS RefSeq effort, a smaller set of sequence accessions from the 28S nuclear large subunit ribosomal gene (LSU) were also collected but not verified. A workflow similar to the ITS record curation process was followed and during continued curation these LSU records have been verified for sequence quality, correct identification, and accurate source data. Close to 500 records (from 800 potential records) representing >100 families from 21 classes were verified and recently released. The 28S dataset can be retrieved from BioProject PRJNA51803 (48).
