Chunk #33 — Results — Nondependent drinking and alcohol dependence alter cell death, proliferation, immature neurons, survival and neurogenesis in the hippocampal SGZ
Cell death was analyzed by probing for two markers, AC-3 to measure apoptotic cell death and Fluoro-Jade C to measure neuronal degeneration. Neuronal degeneration was not observed in the naïve and trained controls. Alcohol-dependent animals showed increased neuronal degeneration in the granule cell layer compared to naïve and trained controls, and nondependent animals (F3,17 = 19.9, p < 0.001, Fig. 4a). Nondependent animals showed increased apoptosis compared with controls and alcohol-dependent animals (F3,17 = 8.5, p = 0.002, Fig. 4b), while apoptosis was not changed in alcohol-dependent animals compared with controls (p > 0.05, Fig. 4b).
