Chunk #32 — Results — Nondependent drinking and alcohol dependence alter cell death, proliferation, immature neurons, survival and neurogenesis in the hippocampal SGZ

Source

Permanent impairment of birth and survival of cortical and hippocampal proliferating cells following excessive drinking during alcohol dependence.

Embedded

yes

Text

Hippocampal sections from naive, naive-trained, nondependent and alcohol dependent animals (Set I, brain tissue collected on day 77) were processed and examined for changes in cell death – apoptosis (AC-3-IR; Fig. 2f) and neuronal degeneration (Fluoro-Jade C-IR; Fig. 2g), cell proliferation (Ki-67-IR; Fig. 2d), immature neurons (DCX-IR; Fig. 2e), survival (Set II, injected with BrdU on day 77 and brain tissue collected on day 96; BrdU; Fig. 2h), neurogenesis (BrdU/NeuN Fig. 2l) and gliogenesis (BrdU/GFAP Fig 2p). Training for the sweetened solution fading procedure did not alter cell death (apoptosis and neuronal degeneration), proliferation and immature neurons.