solution fading procedure did not alter proliferation. Nondependent drinking did not produce a significant change in proliferation compared with drug-naive and trained controls (p > 0.05), whereas alcohol dependence decreased proliferation compared with drug-naive and trained controls (F3,17 = 16.71, p < 0.001, Fig 3c) and nondependent animals (F3,17 = 16.71, p < 0.05, Fig 3c). Alcohol dependence also decreased survival in the mPFC (F2,14 = 6.5, p < 0.05, Fig. 3d). We next evaluated the effects of prolonged nondependent and dependent environment on proliferation in the mPFC (Set II, brain tissue collected on day 96). Surprisingly, although decreased proliferation was observed in dependent animals (Fig. 3c) it was not in the prolonged dependent group (F2,14 = 2.6, p = 0.11, Fig. 3e).
