We used RNA sequencing to examine the effect of binge drinking of ethanol during adolescence on gene expression profiles in the dorsal raphe nucleus (DRN), which has not previously been studied in a binge drinking adolescent model. The DRN is a central serotonergic region of the brain (Waselus et al., 2011). It has serotonergic efferents to the reward centers in the brain and is implicated in stress, anxiety and depression (Hale et al., 2012; Waselus et al., 2011). Adolescent P rats consumed 8–10 g ethanol/kg of body weight/day during the three 1-hour sessions, and did this repeatedly for 5 consecutive days during 3 weeks, with a 2-day withdrawal period after each of the initial two weeks (McBride et al., 2014). This repeated binge drinking of ethanol had a profound effect on gene expression in the DRN, with 30% of the identified genes differentially expressed between the ethanol drinking and control animals (Supplementary Table 1). Key functional groups of genes that were altered in expression included neurotransmitter systems (Table 1), neuropeptide systems (Table 2), ion channels (Table 3), and genes related
