Chunk #37 — Results — KLF12: Regulation of acute functional tolerance to ethanol in C. elegans and gene expression correlation with locomotor activity in mice
We tested a strong loss-of-function allele in klf-3. There was no difference in initial sensitivity between wild-type N2 and klf-3(ok1975) mutants (Figure 4D). While wild-type N2 animals demonstrated normal AFT at 30 minutes, klf-3 mutants failed to develop AFT (Figure 4D, t-test of degree of speed recovery between 10 and 30 min, 400 mM ethanol: N2 vs. klf-3(ok1975), t3=8.99, p<0.001). These data strongly suggest that the transcriptional regulation provided by KLF-3 is required for the development of AFT in worms.
