Chunk #38 — Results — KLF12: Regulation of acute functional tolerance to ethanol in C. elegans and gene expression correlation with locomotor activity in mice
Based on patterns of regulation of mouse Klf12 by ethanol (Wolen et al., 2012), we analyzed correlation between basal Klf12 expression in mouse PFC (GN135), NAc (GN156), VTA (GN228) and whole brain (GN113) datasets and BXD panel phenotypes in GeneNetwork. The strongest correlations observed for basal Klf12 expression in PFC (GN135, probeset 1455521_at) were with locomotor activity 0–5 minutes (trait 11708, correlation rank=1, rho=0.756, p=1.69×10−5, N=22 strains, Figure 5C) and 0–20 minutes (trait 11705, correlation rank=3, rho=0.747, p=2.54×10−5, N=22 strains) after 2.25 g/kg IP ethanol in females (Philip et al., 2010). The first result remains significant and the second falls just below significance after Bonferroni correction. Basal Klf12 expression in mouse NAc (GN156, probeset 1439847_s_at) was positively correlated with AFT (Kirstein et al., 2002) (trait 10348, correlation rank=29, rho=0.560, p=0.003, N=25 strains, Figure 5D). While not significant after Bonferroni correction, this is consistent with the failure to develop AFT in C. elegans klf-3 mutants.
