The distribution of the epigenetic marks within the NSCs is closely related to the chromatin state. Our results indicated that all of the epigenetic marks were located within the nucleus of NSCs, but the DNA methylation associated enzymes/protein (DNMTs, MBD1) were not; the major histone marks and the DNA methylation mark 5-MeC are confined to the nucleus through differentiation, while the enzymes for DNA methylation transfer and the DNA methylation binding protein were more dynamic in different compartments of the nucleus and also expanded into the cytoplasm upon differentiation. The compartmentalization of the epigenetic marks within the nucleus is the key to the heterochromatin and euchromatin states. The examined epigenetic marks were each characteristically distributed: Ac-H4 was ubiquitously distributed in the nucleus, 2me-H3K4 was in the nucleus, but not the presumably nucleoli, and 3me-H3K27 aggregated in granule-like forms. Since the major epigenetic marks mediate chromatin state and accessibility of the gene for transcription, their distribution, and reorganization are likely to have significant outcomes. For instance, 5-MeC distinctly relocated from its originally homogeneous distribution in the nucleus, gradually evacuated nucleolus, and
