For replication analysis of the signals in the 20 CNVRs with evidence for an association in both GWAS samples, we focused on a CNVR-tagging SNP approach (see Materials and Methods section). For two (CNVR 11q11 and CNVR 1p31.1) of these regions, a tagging SNP could be identified, which has been analysed in a third independent sample of 365 obesity trios. In addition, we also screened the 20 CNVRs in a subsample of the 365 obesity trios (281 obesity trios) using additional Affymetrix Genome-Wide Human SNP Array 6.0 data (array-based replication).
