As an additional metric of SCZ-associated behavior, we then assessed sleep and diurnal activity patterns of human SCZ and CTRL glial chimeras, directly comparing mice engrafted with either SCZ (line 52) or control (line 22) hGPCs. We found that mice engrafted with SCZ GPCs were significantly more active than control mice engrafted with normal hGPCs As measured by meters moved per hour, over the course of a 72-hour video-recording (Noldus Ethovision), the SCZ hGPC chimeric mice moved significantly more than their normal hGPC-engrafted controls (2-way ANOVA, F=48.35; p<0.0001) (Figure 6F). Interestingly, while the SCZ-associated increment in activity largely occurred during night-time periods of wakefulness, the SCZ mice also manifested disrupted sleep patterns, as measured by the duration of bouts of inactivity, a surrogate for EEG-validated sleep (McShane et al., 2010; Pack et al., 2007) (Figure 6G). Within the half-hour following the phase transition from dark to light (when mice normally sleep), the CTRL mice had more continuous, uninterrupted patterns of sleep, with an average sleep bout of 511.5 ± 36.4 seconds (8.53 minutes), whereas SCZ mice were asleep for 306.2
