For lentiviral expression constructs, Strep tagged-METTL2A, 2B and DALRD3 as well as 3x-FLAG-tagged DALRD3 and all truncation constructs of DALRD3 were first cloned into the pENTR.CMV.ON plasmid using NheI and NotI restriction sites. The entry vectors were recombined via LR clonase reaction (ThermoFisher) into the pLKO.DEST.puro destination vector to allow for stable integration of the target genes into human cell lines.
