The open reading frame (ORF) for METTL2A and METTL2B was RT-PCR amplified from HeLa human cervical carcinoma cell cDNA, cloned by restriction digest and verified by Sanger sequencing. The cDNA clones for METTL2A and METTL2B correspond to NM_181725.3 and NM_018396.2, respectively. The ORF for DALRD3 was PCR amplified from cDNA plasmid HsCD00338640 (Plasmid Repository, Harvard Medical School) and cloned into either pcDNA3.1-TWIN-Strep or pcDNA3.1-3xFLAG. Truncation constructs were PCR amplified and cloned from the ORF of METTL2A and DALRD3 and cloned into pcDNA3.1-3xFLAG for METTL2A truncation constructs and DALRD3 truncation constructs. Primers used are listed in Supplementary Table 1.
