A total of 46 SNPs at 19 novel adiposity loci, identified through recent GWAS in Europeans, were selected for replication in our African-American cohorts. Selection criteria included key SNPs that reached genome-wide significance (P<5 × 10−8) and nearby reported SNPs showing nominal association in these previous studies.10,13-20 For loci with multiple associated SNPs, the pair-wise linkage disequilibrium (LD) D’ and r2 in HapMap Phase II Yoruba were assessed using Haploview (http://www.broadinstitute.org/haploview). Only representative SNPs with r2<0.8 were selected for genotyping (Supplementary Table 1). Cohorts 1 and 2 were genotyped at the Center for Inherited Disease Research, using 1 μg of genomic DNA on the Affymetrix Genome-wide Human SNP array 6.0 (Affymetrix, Santa Clara, CA, USA). Genotypes were called using Birdseed version 2, APT 1.10.0 (Broad Institute, Cambridge, MA, USA), and samples were grouped by DNA plate to determine the genotype cluster boundaries. Genotyping of samples from cohorts 3 to 6 was performed using the iPLEX Sequenom MassARRAY platform (San Diego, CA, USA). The minimum and average SNP call rates for all cohorts were 95% and 98.1%, respectively. The average genotype
