Primate samples had five drinking categories: controls (alcohol naïve), low drinkers, high drinkers, binge drinkers, or very high drinkers. To reduce multiple testing, we collapsed the top drinking categories into a single alcohol group and compared this group to the lowest drinking category (naïve controls in the NAc or the low drinking category in the PFC and CEA; note PFC and CEA samples had no naïve alcohol group). We removed samples with a normalized RNA-seq read count below two standard deviations of the group mean, which left a total of 81 primate brain samples (nNAC = 23; nCEA = 28; nPFC = 30). All primate procedures were reviewed and approved by the Oregon National Primate Research Center IACUC and were in accordance with the Guide for the Care and Use of Laboratory Animals as well as the NIH guidelines for the care and use of laboratory animal animals. For more information on the primate RNA-seq data see Iancu et al.15 and Walter et al.16. Briefly, paired-end and stranded RNA libraries were prepared via the TruSeq RNA sample preparation kit. PFC and CEA data were ribo-depleted (RiboZero Gold rRNA depletion) and sequenced on the Illumina HiSeq 2000, whereas NAc data were
