Chunk #10 — Materials and methods — Samples — RNA-seq

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Genome- and transcriptome-wide splicing associations with alcohol use disorder.

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yes

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and Walter et al.16. Briefly, paired-end and stranded RNA libraries were prepared via the TruSeq RNA sample preparation kit. PFC and CEA data were ribo-depleted (RiboZero Gold rRNA depletion) and sequenced on the Illumina HiSeq 2000, whereas NAc data were PolyA selected and sequenced on the Illumina HiSeq 2500. In total, the primate brain samples had an average of 43,133,419 paired-end reads (s.d. = 14,096,098).