Chunk #6 — Materials and Methods — Measurement of superoxide dismutase activity

Source

Social isolation exacerbates schizophrenia-like phenotypes via oxidative stress in cortical interneurons.

Embedded

yes

Text

Medial PFC tissue was dissected and homogenized in cold PBS (10% w/v) with a tissue sonicator. The supernatant was assayed for superoxide dismutase (SOD) using a determination kit (Sigma-Aldrich) after centrifugation at 21,000 g for 10 min at 4°C as per the manufacturer’s instructions.