Chunk #26 — Methods — In vitro optical stimulation and fast-scan cyclic voltammetry

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Selective activation of cholinergic interneurons enhances accumbal phasic dopamine release: setting the tone for reward processing.

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4 weeks after virus injection, mice were killed by decapitation, the brain quickly removed and incubated in modified Krebs buffer. Coronal slices containing the NAc (250 μm thick) were obtained, incubated and then transferred to the recording chamber, perfused at 1 ml/min with 34°C oxygenated Krebs buffer.