Chunk #27 — Methods — In vitro optical stimulation and fast-scan cyclic voltammetry

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Selective activation of cholinergic interneurons enhances accumbal phasic dopamine release: setting the tone for reward processing.

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Using a cylindrical carbon fiber, voltammetric recordings (versus an Ag/AgCl reference electrode) were performed using Demon Voltammetry and Analysis Software. Optical and electrical stimulation were delivered through an optical fiber in apposition with the brain slice and through a bipolar tungsten electrode in contact with the slice, respectively.