After incubation, brain striatal slices were transferred to the recording chamber, superfused with artificial cerebrospinal fluid and maintained at 30 ± 1° C. Current- and voltage- clamp recordings of CINs were performed in unmodified aCSF, while voltage clamp recordings of MSN EPSCs were performed in aCSF containing 50 μM picrotoxin to block GABAA-mediated currents. Optical stimulation was delivered via the epifluorescence light path. Clampex 10.3 software was used for data acquisition.
