To determine if Lmo3 is involved in behavioral responses to ethanol, we tested transgenic founder mice expressing shScr or shLmo3.8 for their response to the acute sedative /hypnotic effect of ethanol in the LORR test. We observed a significant negative correlation between expression levels of Lmo3 and sedation time at a dose of 3.2 g/kg ethanol (Fig. 4A, R = −0.663, p = 0.019), suggesting that reduced expression of Lmo3 leads to increased sensitivity to the sedating effect of this dose of ethanol. Sedation time at higher doses of ethanol (3.6 and 4 g/kg) was not significantly correlated with Lmo3 expression (data not shown). The same animals were tested 2 weeks after the completion of the LORR test for voluntary ethanol consumption using a 2-bottle choice protocol. Significant positive correlations were observed between Lmo3 expression and ethanol consumption at 6% (Fig. 4B, R = 0.719, p = 0.008), 10% (Fig. 4C, R = 0.691, p = 0.013), and 14% ethanol (R = 0.591, p = 0.043, data not shown). Although not significant, we observed a trend correlating Lmo3 expression with
