In the Acb-sh, several signaling pathways appear to be activated by chronic adolescent binge-drinking of P rats, as indicated by the number of genes up-regulated compared to the number down-regulated in the cAMP-mediated signaling (7:2 ratio), glucocorticoid receptor signaling (7:2 ratio), RAR activation (5:1 ratio), G-protein coupled receptor signaling (8:3 ratio), and protein kinase A signaling (7:2 ratio) pathways (Table 4). The more global WGCNA was consistent with the IPA and provided support for the observed increase in G-protein coupled receptor signaling and alterations in synaptic transmission. Although the WGCNA indicated many different biological categories were altered by adolescent alcohol drinking in the CeA, there was only one signaling pathway listed, i.e., transmembrane receptor protein tyrosine kinase (Table 5). Overall, these results suggest that in the Acb-sh, and to a lesser extent in the CeA, adolescent alcohol drinking by P rats is producing significant developmental alterations in intracellular signaling pathways.
