To further test the utility of iPSC-derived cells, we conducted a side by side whole genomic comparison of iPSCs and hESCs during dopaminergic differentiation using the defined medium protocol described earlier. Cells were harvested at three stages (ESC/iPSC, NSC, and dopaminergic neuron), and a transcriptome analysis was performed using Illumina arrays and pathways analysis software as described in “Materials and Methods” section and previously [18]. hESC data presented in this article is similar to previous analysis for the same population at a different passage number allowing for future comparisons of discrete data sets. The current analysis was limited to this data set (A total of nine samples: undifferentiated hESC line H9, iPSC lines MR31 and MMW2, NSCs derived from H9, MR31 and MMW2, and day 31 dopaminergic neurons derived from H9, MR31, and MMW2).
