All three traits – AD-CrC, MAXDRINKS, and flushing – showed GWS association in the total (meta-analyzed) sample with variants near ALDH2, the lead SNPs mapping to an LD block containing rs671 (Table 2). The associations differed in statistical significance, as follows: Flushing, lead SNP PTPN11*rs143894582; Sample 1 (GSA), 8.33 × 10−8; Sample 2 (MEGA), 2.76 × 10−8; pmeta=2.01 × 10−14 (Figure 1). MAXDRINKS, lead SNP rs149212747; Sample 1 (GSA), 9.03 × 10−9; Sample 2 (MEGA), 4.59 × 10−5; pmeta=1.61 × 10−12. And AD-CrC, lead SNP rs149212747; Sample 1 (GSA), 1.54 × 10−5; Sample 2 (MEGA), 6.96 × 10−6; pmeta=5.80 × 10−10 (Figure 2). The only trait without GWS in either of the separate samples taken individually was AD-CrC – although this trait reached high statistical significance in the meta-analysis. Manhattan and QQ plots for flushing (individual samples) and MAXDRINKS (individual samples and meta-analysis) are shown in Supplement Figures 2–4. Individual-sample level Manhattan and QQ plots for AD-CrC are shown in Supplemental Figure 5. (Rs671 was directly genotyped in both samples. Rs143894582 was imputed in both samples, with imputation score 0.801
