To determine the extent to which modules are preserved across brain regions we employed two complementary procedures. First we utilized the WGCNA modulePreservation function to check whether modules detected in one region show increased coexpression/connectivity in the other regions, recognizing that they might be distributed across different modules even if preserved. A second measure of module preservation was computed based on the gene overlap between all module pairs in all three regions, which is denoted as tabulation-based module preservation in the modulePreservation WGCNA function (Langfelder et al., 2011).
