A total of 85 males and 42 females (n=127) completed the alcohol sensitivity sessions, consented to genetic sampling, and had adequate material for genotyping; Thirty-six of these subjects were included in a previous study (Uhart et al., 2013), which was completed using the same procedures, at the same site, with the same investigative team, and the same inclusion/exclusion criteria. Procedures for genotyping are in Supplemental Methods B. Based on known race/ethnicity differences in genotype frequency distributions (Chen et al., 2012), we determined genetic ancestry for each subject using AIM determined from a panel of 96 markers using GoldenGate Genotyping Assay (Mahon et al., 2013) and a set of 22 microsatellite markers, with allele 1 and allele 2 plus Duff-ag, which have a high efficiency at clustering individuals into population subgroups. Data were analyzed using Structure (v.2.3.2) (Stephens et al., 2012), and assumed a model with the possibility of admixture and correlated allele frequencies between populations; all other parameters were kept at their default values. In order to increase the accuracy of inferred genetic ancestry, the AIMs were used to generate
