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Chunk #20 — 3. Results

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Differential effects of ethanol in the nucleus accumbens shell of alcohol-preferring (P), alcohol-non-preferring (NP) and Wistar rats: a proteomics study.
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There were 875 proteins in category 1 (peptide ID confidence > 99%; with 2 independent peptides) that were identified in the ACB-shell samples of P, NP and W rats (see supplemental table A for complete list of category 1 proteins; see supplemental table B for list of all peptides that were used to identify each priority 1 protein). There were no significant differences in protein expression levels in the ACB-shell between the saline treated (control) P and NP rats. Comparison of the saline treated P and W rats yielded significant differences (q < 0.25; P < 0.015; fold change ≥ 1.15) in 2 proteins: fumarate hydratase mitochondrial precursor and fumarate hydratase-1. Both proteins had higher values in the ACB-shell of P than W rats. These same 2 proteins (and no others) also had higher expression levels in the ACB-shell of NP than W rats.