Figure 1 shows the total ion chromatogram (TIC) from the 1st, 30th and 58th injection to demonstrate that the overall quality of the chromatograms was maintained from the 1st to the 58th injection. Figure 2 shows the extracted ion chromatogram (XIC) for a single peptide from guanine nucleotide-binding protein G(i), alpha-1 subunit (Gnai1) to illustrate how the area under the curve (AUC) was determined for two different samples. Table 1 shows the normalized average AUC values for the 5 peptides used to quantify levels of Gnai1 in the 6 experimental groups.
