the most significant effect of ethanol on gene expression among all inflammatory markers measured was found in the hippocampus, where we also observed the most infiltration of CCR2+ peripheral macrophages. Tables 3, 4, and 5 provide the mean and standard deviation for each gene and group in the cortex, hippocampus, and cerebellum as well as p value comparisons between pair- and alcohol-fed mice (PF vs EtOH) as well as alcohol-fed and CVC-treated alcohol-fed mice (EtOH vs 6wk CVC; EtOH vs 3wk CVC). Significant differences are denoted with an asterisk (*) and are offset in the column (Tables 3, 4, and 5). Table 3Gene expression profile of the cortexCortexPFEtOHPF vs EtOHEtOH + 6wk CVCEtOH vs 6 weeksEtOH + 3wk CVCEtOH vs 3 weeks, p valueMeanStdevMeanStdevp valueMeanStdevp valueMeanStdevNanostring C1qa1.0210.2421.4720.5190.0890.7510.185*0.0120.7500.265*0.015 C1qb1.0150.1961.0160.2920.9960.9590.2720.7970.8820.2430.426 Ccl111.1560.74314.5212.660.0734.6263.510*0.0363.3333.0570.062 Daxx1.0200.2191.3310.3030.0800.7510.108*< 0.0010.8900.294*0.038 Gnas1.0170.2181.1670.2790.3420.7340.068*< 0.010.6870.173*< 0.01 Hmgn11.0040.0991.1670.3070.2471.0860.1570.5080.9600.2440.243 Hspb11.0170.2041.2750.143*0.0421.0340.2380.1240.9370.237*0.021 Il231.5841.5470.9260.6570.4012.4842.3260.2222.4300.941*0.015 Itgb21.1030.4980.8330.4470.3940.3840.2030.1230.7260.6210.771 Map3k91.0680.4590.8250.4220.3890.6450.4270.4330.5760.1800.219 Mapk11.0750.4991.2580.2690.4820.8200.3970.1080.8430.267*0.031 Mef2a1.0190.2050.9130.2100.4201.0930.3840.4021.0720.3910.437 Myd881.0170.2000.9680.2440.7201.0960.2630.5960.9310.1080.750 Plcb11.0020.0700.8940.1880.2230.740.0900.2310.8240.1260.474 Tgfβ11.0230.2361.4740.4220.0520.8390.450*0.0380.6570.194*< 0.01Proinflammatory Tnfα1.1350.6512.5971.6940.0812.0851.1960.5300.7820.796*0.043 Il1β1.2310.7571.190.4030.9160.6670.2350.0611.1450.6670.899 Il171.1800.6960.8230.2790.3121.9750.695*0.0181.6761.1700.149 Tlr41.0230.2361.2090.4920.4301.0240.4090.6781.0130.3090.439 Cox21.0100.1531.2650.2290.0541.0960.3170.4341.2340.1390.788 Ym11.0930.41222.4013.11*< 0.018.8499.192*0.0138.1837.048*0.041CCR2/5 network Ccl21.1870.6330.9600.2210.5242.2270.552*< 0.011.6841.4580.364 Ccl31.3110.8212.0901.5270.3071.9091.7160.7641.5491.0330.502 Ccr21.0650.4020.7640.1650.2001.2141.7180.6231.2560.5640.134 Ccr51.0190.2231.5300.412*0.0271.0810.3780.0601.2960.5180.436Gene expression was measured by qPCR. Data are mean or standard deviation (Stdev), n = 5–6 mice/group*p < 0.05 and significant p values are offset from non-significant valuesTable 4Gene expression profile of the hippocampusHippocampusPFEtOHPF vs EtOHEtOH + 6wk CVCEtOH vs 6 weeksEtOH +
