Dependent measures of interest that are typically sensitive to the systemic effects of cannabinoids include locomotor activity, antinociception, catalepsy, and hypothermia (Little, et al 1988). To assess locomotor behavior, rats were placed in clean plastic cages (28 × 16 cm) inside sound-attenuating chambers and distance traveled was recorded for 5 min and analyzed by the ANY-maze (Stoelting, Wood Dale, IL) video tracking system. Antinociception was assessed in the tail-flick test as previously described previously (D'Amour and Smith 1941). To minimize tissue damage, a maximum cut-off latency of 10 s was used. Catalepsy was determined using the bar test (Pertwee and Wickens 1991), in which the front paws of each subject were placed on a rod (0.75 cm diameter) that was elevated 4.5 cm from the bench top. The duration of time that the rat remained motionless (with the exception of respiratory movements) with their front paws on the bar for 10 s was scored. Rectal temperature was determined using a telethermometer (Physitemp Instruments, Inc., Clifton, New Jersey) by inserting a thermocouple probe 4.5 cm into the rectum. The rats were
