Our protocol efficiently generates cultures containing an enriched population of O4+‐ and MBP+‐oligodendrocytes within 1 week of hPSC‐OPC differentiation and is of an equivalent duration to that recently reported 17. The ability to propagate OPCs with FGF2 and PDGFα treatment and the reproducibility of the method across multiple hPSC lines confirms the suitability of the protocol for in vitro disease modeling studies. Critical to electrophysiological studies is the observation that >80% O4+‐oligodendrocytes co‐express MBP, but < 10% O4+‐cells coexpress PDGFRα. This allows live‐staining prior to electrophysiological analysis to identify and discriminate between OPCs and oligodendrocytes.hPSC‐derived OPCs display outwardly rectifying membrane currents and differentiation to oligodendrocytes results in a linearization of the membrane currents. These properties have been widely described in rodent oligodendroglial counterparts 23, 24, 25, in vitro mouse PSC‐derived OPCs 48 and integrated mouse PSC‐derived glial restricted progenitor cells that contain a proportion of OPCs 49, 50. These data indicate that comparable shifts in ion channel expression are occurring throughout human oligodendrogenesis in vitro. Our data show that the expression of voltage‐gated K+ channels, I A and I K,
