Mice were treated and tested as shown in Figure 1. Groups of mice were treated during adolescence (P28-37; Figure) and during adulthood (P88-97, Figure 1). Mice were given either water or ethanol (5g/kg, 25% ethanol w/v) intragastrically once per day for 10 days. Adolescent ethanol (AE) treatment resulted in an average blood alcohol level of 287.7 mg/dL ± 36.8 (mean±SEM) measured 1 hour after the last ethanol administration. This is similar to blood alcohol levels that we have observed previously in young adult mice (P56-65) treated with ethanol (5 g/kg/day i.g), where we observe blood alcohol levels of 316 mg/dL ± 11 (Qin et al., 2008). Binge ethanol treatment caused a delay in the adolescent growth spurt. In control mice, an increase in weight occurred from P28 to P32 (15.8 g to 17.5 g, 11%) followed by a plateau in weight from P32-P36 and another increase in weight from P36 to P49 (3.5 g, 19% increase) (Supplemental Figure 1). Mice that received the ethanol binge during adolescence were different from controls in that they did not experience a weight gain
