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Chunk #16 — Materials and Methods — Genotyping

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The effect of alcohol priming on neural markers of alcohol cue-reactivity.
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Saliva samples were collected using Oragene saliva collection kits (Kanata, Ontario, Canada) and sent to the UCLA Genotyping and Sequencing Core for genotyping. Polymerase chain reaction was performed on Applied Biosystems (Carlsbad, CA) dual block PCR thermal cyclers. Single-nucleotide polymorphisms were run on an AB 7900HT Fast Real-Time PCR System and analyzed using the Sequence Detection Systems software version 2.3. Genotypes were automatically scored by the allele calling software, and each genotype was verified by visual inspection.