Associations between CD and the eight SNPs in CNR1 in the EA family sample are shown in Figure 2. rs806368 (SNP8) showed the most significant association signal (P=0.019). The second most significant association signal was for rs806371 (SNP6, P=0.038), which showed almost as high a δ peak as SNP8 in the fine-mapping analysis in our initial study (δ is a measure of LD used in fine-mapping analysis) (Zuo et al 2007). SNP6 is located within the SNP8-containing haplotype block and is correlated with SNP8 (D’=0.84, r2=0.49). Thus, the CD association signal at SNP6 is consistent with the association between SNP8 and CD, and also suggests that SNP6 may help to capture the association signal of SNP8 locus. The third most significant CD association signal was seen for rs6454674 (SNP3), though this did not reach statistical significance (P=0.060). No other association signal was observed.
