Chunk #49 — Online Methods — Genome-wide survival association study datasets — (4) Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE)
the FHS Original and Offspring participants are white/Caucasian. FHS participants had DNA extracted and provided consent for genotyping in the 1990s. All available eligible participants were genotyped at Affymetrix (Santa Clara, CA) through an NHLBI funded SNP-Health Association Resource (SHARe) project using the Affymetrix GeneChip ® Human Mapping 500K Array Set and 50K Human Gene Focused Panel ®. In 272 persons, small amounts of DNA were extracted from stored whole blood and required whole genome amplification prior to genotyping. Cell lines were available for most of the remaining participants. Genotyping was attempted in 5,293 Original and Offspring cohort participants, and 4,425 persons met QC criteria. Failures (call rate <97%, extreme heterozygosity or high Mendelian error rate) were largely restricted to persons with whole-genome amplified DNA and DNA extracted from stored serum samples. In addition, since the persons with whole genome amplified DNA represent a group of survivors who may differ from the others we included whole genome amplified status as a covariate in FHS analyses. After exclusion of prevalent dementia, dementia other than AD, and missing values, a sample of 2,208 participants was available for this project. The FHS component of this study was approved by the Institutional Review Board
