All experiments were performed on Sprague Dawley (Harlan, IN) male rats that were individually housed in a temperature-controlled (20–22°C) environment with a 12-h light/dark cycle. Subjects were maintained on a food-restricted diet in order to sustain body weights of approximately 85% of free-feeding weight. Water was available ad libitum. All animal protocols were approved by the Virginia Commonwealth University Institutional Animal Care and Use Committee and were in concordance with the Guide for Care and Use of Laboratory Animals (Institute of Laboratory Animal Resources, 1996).
