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Chunk #13 — METHODS — Microarray analysis of brain tissues

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Ethanol treatment of lymphoblastoid cell lines from alcoholics and non-alcoholics causes many subtle changes in gene expression.
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To determine which genes were expressed both in the LCLs and in the brain, we matched gene symbols associated with the probe sets on the two different arrays. We were able to match 24,668 of the 26,814 genes that were detectably expressed in at least one group of LCL samples (on the Affymetrix HG U133 Plus 2 GeneChips®) with genes on the Human Gene 1.0 ST arrays on which the brain samples were analyzed.