Ethanol-responsive and basal expression of Clic4 in mouse PFC(A) qRT-PCR analysis of basal (Saline) and ethanol-responsive (EtOH, 4 g/kg, 4 hr) Clic4 expression in DBA2/2J mice. Expression of Clic4 is elevated after ethanol treatment, validating prior microarray results (Kerns et al., 2005) . (B and C) Pearson correlation of Clic4 basal expression in PFC (x-axis) of BXD recombinant inbred lines (numbered points) with ethanol-induced locomotor activity (GeneNetwork trait ID 11962 (Philip et al., 2010)) (B) and initial sensitivity to ethanol-induced rotarod ataxia following first of five injections (onset of ataxia brain ethanol threshold, mg ethanol/g brain – GeneNetwork ID 10144 (Gallaher et al., 1996)) (C). Scattergrams were generated in GeneNetwork (www.genenetwork.org) using the VCU PFC saline database (Wolen and Miles, unpublished). B6 and D2 strains were not tested in experiments shown in panel B
