The unique contribution of curated eukaryotic RefSeq transcript records is that they integrate functional information with a reference sequence. RefSeq curation staff adds gene summaries, nomenclature, transcript variant text, gene and sequence attributes, and functional features that are available on the RefSeq record and/or through the Gene resource (http://www.ncbi.nlm.nih.gov/gene). In the past year, RefSeq staff have pursued several in-depth annotation projects, some of which are briefly described in the following paragraphs, to add functional data to specific sets of genes where computational tools are unable to accurately represent biological knowledge. These projects include annotation of antimicrobial peptides, endogenous retroviruses, replication-dependent histones, regulatory uORFs, and antizymes.
