Sequenom technology was utilized to validate the methylation microarray findings (see Methods). An example of alignment between Sequenom and MeDIP for four genes is shown in Figure 7. Decreased methylation of Elavl2 (Embryonic lethal, abnormal vision, Drosophila-like 2; a.k.a. Hu antigen B), Sox21 (SRY-box containing gene 21), Sim1 (single minded homolog 1), Hist1h3d (histone cluster 1, H3d), Igf2r (insulin-like growth factor 2 receptor), and Nlgn3 (neuroligin 3), and increased methylation of Cyp4f13 (cytochrome P450, family 4, subfamily f, polypeptide 13) was confirmed using Sequenom technology. Furthermore, decreased methylation of Elavl2 and Sox21 corresponded with increased gene expression, while Nlgn3 and Igf2r expression was not altered significantly. Cyp4f13 expression data was not available.
