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Chunk #18 — Materials and Methods — Smoking-related diseases — Peripheral arterial disease

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A rare missense mutation in CHRNA4 associates with smoking behavior and its consequences.
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Iceland The study population has been described previously.2 Briefly, Icelandic individuals with PAD were recruited from a registry of individuals diagnosed with PAD at the Landspitali University Hospital, during the years 1983–2011. The PAD diagnosis was confirmed by vascular imaging or segmental pressure measurements. To study early-onset PAD we considered subjects diagnosed with PAD before reaching age 65.