Adolescent exposure to ethanol also tended to alter responsivity in a conditioned place preference paradigm in young adulthood. Adolescent male rats were chronically exposed to saline or ethanol (0.75 g/kg/ip) during adolescence (PND 30–50) followed by a 14-day washout period and were conditioned with saline or ethanol (0.75 g/kg/ip) in young adulthood in a two-chambered apparatus (Maldonado, Badanich and Kirstein, unpublished data). In young adulthood (PND 65–70), rats were conditioned with saline or ethanol. Control rats had saline paired with each environment for a total of eight saline conditioning trials. For rats conditioned with ethanol in adulthood, four pairings with saline and four pairings with ethanol occurred. As shown in Figure 3, adolescent rats that were pretreated with ethanol and conditioned with ethanol in young adulthood spent significantly more time in the ethanol-paired chamber relative to those adolescent rats that were pretreated with saline and conditioned with ethanol in young adulthood. Additionally, adolescent rats that were treated with ethanol and subsequently conditioned with ethanol in young adulthood demonstrated a greater amount of time spent in the ethanol-paired chamber relative to
