Alcohol-induced methylation alterations in the chromosomal landscape were observed, with a higher overall number of methylation alterations (>200) in chromosomes 7, 10 and X chromosomes (Fig. 6), which are known to contain many imprinted genes and genes prone to silencing. Furthermore, a greater number of methylation alterations were seen in ALC-NTO than in ALC-NTC groups, e.g., 201 increased methylation changes were observed in chromosome 10 of ALC-NTO, but only 5 such changes were seen in ALC-NTC (Fig. 6). In the X chromosome of ALC-NTO, 267 increased methylation changes were found compared to only 17 in ALC-NTC (Fig. 6).
