Transfected with Nrf2 siRNA, Nrf2 protein expression declined 86% in 24 h and 84% in 48 h, respectively (Figures 4A,B). Transcription of Nrf2 in HepG2 cells declined 90.10% in 24 h and 91.26% in 48 h, respectively (Figure 4C). Twenty-four hours after pcDNA3-EGFP-C4-Nrf2 was transfected in HepG2 cells, Nrf2 protein (Figures 4D,E), and mRNA (Figure 4F) expression was multiplied by 2.01 and 10.88, respectively. Forty-eight hours after pcDNA3-EGFP-C4-Nrf2 was transfected, Nrf2 protein (Figures 4D,E) and mRNA (Figure 4F) expression was multiplied by 1.89 and 10.84, respectively. GSTA1 mRNA relative expression showed an elevation of 528% in cells transfected with pcDNA3-EGFP-C4-Nrf2 and was decreased by 43.1% in cells transfected with Nrf2 siRNA (Figure 4G). In cells transfected with NC plasmids (NC siRNA or pcDNA3-EGFP-C4), Nrf2, and GSTA1 expressions showed no statistical difference compared to the control cells (Figure 4). CYP2A6 mRNA and protein expressions were increased by LA and ALA treatment in HepG2 cells transfected with functional plasmids or NC plasmids. HepG2 cells were examined for mRNA and protein expression levels in the presence or absence of LA and ALA treatment,
